PASCAL - Pattern Analysis, Statistical Modelling and Computational Learning

An analysis of the feasibility of short read sequencing
Nava Whiteford, Niall Haslam, Gerald Weber, Adam Prügel-Bennett, Jonathan W. Essex, Peter L. Roach, Mark Bradley and Cameron Neylon
Nucleic Acids Research Volume 33, Number 19, e171, 2005.


Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20–30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1.

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EPrint Type:Article
Project Keyword:Project Keyword UNSPECIFIED
Subjects:Theory & Algorithms
ID Code:1777
Deposited By:Adam Prügel-Bennett
Deposited On:28 November 2005